How It Works
Cryo-Fluorescence Tomography (CFT) is an ex vivo molecular tissue imaging technique that allows the visualization of cell clusters, isolated organs, and whole animals. Emit Imaging’s suite of software and instrumentation, takes CFT to the next level. Built on upgradeable technology, Emit Imaging delivers transformational innovation. A technology powerhouse.
Xerra is a powerhouse.
Xerra houses a 12MP high resolution camera, a 10 micron slicer, 6 lasers, 7 interchangeable emission filters, a refrigerated storage, and powerful software. In. One. Single. Platform.
Xerra is automatic.
Xerra automatically slices, images, reconstructs or renders in 3D and analyzes data sets. By performing as many as 1000 scans per specimen, Xerra extracts exponential volumes of data from a single sample.
Xerra is versatile.
Supports 3D fluorescence imaging from 470 nm to 850 nm, including visualization of a wide range of fluorophores (Alexa family, BODIPY family, FITC, Cy family, Rhodamine family, SYBR family and many more). Xerra can accommodate a wide range of sample sizes – from cell clusters to whole animals.
Xerra is cost-effective.
Xerra has a one-stop automation that replaces many weeks of lab work, tissue processing, microtome slicing, slide preparation, and expensive consumables.
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Xerra Workflow
How To Prepare Samples For Xerra
1. Whole tissue is embedded in the block.
2. The block is mounted in the system and Xerra is started.
3. Camera images the block in Fluorescent Light and White Light.
4. Tissue is sliced and imaged, hundreds of times. Sections can be collected at any point.
5. Image stack is corrected and reconstructed into 3D model and analyzed.
Optimized Functionality for Preclinical Research
Configurable White Light and Fluorescence Imaging
- Fluorescence excitation via narrow band for specific dyes and stains
- High power lasers capture precise image for wavelengths from 470-780 nm
- Narrow band emission acquisitions for clear images in wavelengths from 500-850 nm
- Auto exposure minimizes color bleaching and oversaturation
- Bracketing for large dynamic range data collection
Flexible Sample Imaging Parameters
- Maximum Field of View: 24 cm x 14 cm
- Minimum Field of View: 8 cm x 5 cm
- In plane Resolution: 20 μm to 100 μm
- Section Thickness: 10 μm to 50 μm
Onboard Data Handling and Study Monitoring
- Onboard computer and touch screen displays
- Real-time slice-by-slice quality analysis (QA) to ensure data integrity
- Switch any time between automatic and manual sectioning
- Refrigerated chamber operating at -20° C
Image Analysis and Quantification
- 3D reconstruction software
- VivID™ full resolution data viewing package
- Compatible with various software packages for analysis
- Can be integrated iPACS® for data management
