Home  /  Cryo-Fluorescence Tomography  /  Xerra™
Instrumentation

Xerra™ imaging platform

A fully automated CFT workflow to advance discoveries in biological and drug research.

Contact Us
EMIT Imaging's flagship platform

Run whole-body samples in-house

Xerra™ is EMIT Imaging's flagship preclinical imaging platform, available for purchase. Xerra is designed to advance discoveries in biological and drug research and fully automates the CFT workflow, providing 3D images with high-resolution and high-sensitivity. This enables researchers to easily run samples in-house and obtain unmatched visualization of drug distribution and protein expression in whole-animals.

Xerra provides:
Automated CFT workflow
Anatomical, RGB white-light images registered with fluorescence images
Capable of multiplexing
5 magnifications: 20-55 µm pixel resolution
6 excitation lasers: 470 to 780 nm
7 emission filters: 500 to 850 nm
Xerra™ Brochure →
Xerra imaging platform instrument
Xerra excitation lasers and filters
Xerra excitation lasers and emission filters chart
The workflow

How CFT works

Step 1

Sample preparation

Cryo-preserve and embed the sample in OCT.

Step 2

Image + section

High-resolution, high-sensitivity white-light and fluorescence images are acquired. The block is sectioned and 20-55 microns are removed from the surface. The process repeats until the whole sample is sectioned and imaged.

Step 3

Output

3D image stacks are generated. White-light and fluorescence data can be overlaid.

Why Xerra

Research advantages

High resolution

High, near-cellular resolution down to 20 µm.

High sensitivity

Nanomolar sensitivity, comparable to nuclear medicine.

High throughput

Several samples in the same block means a large volume of tissue imaged.

Complementary

Fits into any experiment, complementing tissue and radiology imaging.

Simplified

No fixation, perfusion, clearing, or radiolabeling required.

Multiplexing

6 lasers and 7 filters for multiplexed applications.

The details

Specifications

High resolution
20 µm to 55 µm isotropic voxels depending on FOV
True 3D
Reconstruct whole-body and large tissue data from white-light and fluorescence images
Automated
Turn-key workflows for image acquisition; automated reconstruction of 2D slices into 3D; RGB white-light registered with high-sensitivity fluorescence, capable of multiplexing
Simplified
No fixation, perfusion, clearing, or radiolabeling. For CFT services: sac, freeze, and ship to EMIT. Frozen samples store for months to years. Compatible with most common fluorophores
Multiplexing
6 excitation lasers (470-780 nm) and 7 emission filters (500-850 nm)
Quantification
Fully characterized fluorophores with optimized laser power, exposure time, and subsurface signal analysis. Signal is surface-weighted and linear
Exposures
Recording at variable exposure times (5, 50, 500, 1500, 25000 milliseconds)
Optical field of view
Max: 24 cm x 14 cm; Min: 8 cm x 5 cm
Working volumes
FOV A: 20 µm, 8x6x4 cm, tissue. FOV B: 30 µm, 10x8x5 cm, 1 mouse. FOV C: 35 µm, 14x11x6 cm, 3 mice. FOV D: 45 µm, 18x14x8 cm, 4 mice. FOV E: 55 µm, 24x14x10 cm, 5 mice or 1 rat
Section thickness
20 µm to 50 µm
Chamber temperature
Refrigerated chamber, -20°, with auto-defrost functionality
Data management
Touch-screen display, real-time slice-by-slice QA, WIFI connectivity or USB A and C data ports; compatible with various analysis software packages

Bring CFT in-house with Xerra™