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Whole-body biodistribution, resolved in 3D.

Cryo-Fluorescence Tomography maps whole-body fluorescence and anatomy in 3D, providing precise anatomical localization of a drug, vector, or fluorescent reporter.

The technology

What is Cryo-Fluorescence Tomography?

Cryo-Fluorescence Tomography (CFT) is a transformative 3D approach to imaging drug distribution and protein expression in whole animals. EMIT Imaging offers both instrumentation and services via our imaging platform, Xerra™, a high resolution and high sensitivity preclinical imaging tool designed to advance discoveries in biological and drug research.

CFT can be easily integrated into your existing in vivo imaging workflows, providing definitive anatomical and molecular insights into:

Whole-body drug distribution and delivery
Screening candidate drugs and delivery systems
Whole-body therapeutic protein expression
Multiplexed co-localization of drug with targets
On and off target tissue identification
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The EMIT Viewer is the web version of our visualization software. Open an example dataset to explore CFT for yourself, nothing to download and nothing to install.

Resources

Webinars, videos, and downloads

Why CFT

Benefits of Cryo-Fluorescence Tomography

CFT expands preclinical imaging capabilities by bridging the gap between cellular microscopy (imaging small tissue volumes at high resolution) and current whole-body techniques such as MRI, PET, and SPECT (imaging larger tissue volumes with lower resolution and sensitivity).

Cryo-Fluorescence Tomography

CFT fluorescence 3D reconstruction
3D CFT
CFT anatomical white-light section
Anatomical
2D CFT
3D anatomical + molecular High resolution High sensitivity

2D Fluorescence

2D fluorescence mouse image
2D surface image Low resolution Limited anatomical info Signal highly attenuated

SPECT/CT

SPECT/CT fusion mouse image
Low resolution Limited anatomical info Requires radioactivity

Cryo-Fluorescence Tomography Instrumentation & Services

Services

CFT fee-for-service research

EMIT Imaging offers CFT fee-for-service research performed in-house, at our state-of-the-art labs in Boston, MA and Baltimore, MD. Simply freeze samples, ship them directly to us, and let EMIT handle the rest.

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Instrumentation

Xerra™ imaging platform

Xerra™ is EMIT Imaging's flagship imaging platform. Xerra fully automates the CFT workflow, enabling you to run samples in-house with ease, and provides unmatched visualization of on and off target drug distribution and protein expression in whole-animals.

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Applications

Preclinical Research Application Areas

Drug Discovery & Delivery

CFT is an important tool to monitor drug distribution, pharmacokinetic, and pharmacodynamic studies.

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Oncology & Immunotherapy

CFT can play a key role for studying tumor models including microenvironments, tumor heterogeneity, metastatic spread, and expression of specific biomarkers.

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Gene & Cell Therapy

CFT and Xerra™, a 3D imaging technology, allows whole-body detection and visualization of vector biodistribution and vector-mediated gene expression, in high resolution and with high sensitivity.

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Neuroscience

The 3D CFT maps of fluorescence reporters can aid in visualization of cell tracking, drug delivery, and pharmacodynamics in the brain.

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Antibody-Drug Conjugates

CFT provides whole-body visualization of ADC biodistribution, helping characterize on and off target payload delivery and linker stability.

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