Multiplexed CFT

Multiple targets. One whole animal. Resolved in 3D.

Explore multiplexed fluorescence channels together with anatomy across the whole body in the EMIT Viewer.

Whole-body 3D Anatomy and molecular signal in the same animal
High resolution Bridging cellular microscopy and whole-body imaging
High sensitivity Detects on and off target distribution
Natick, MA and Owings Mills, MD Fee-for-service labs and Xerra™ manufacturing
Two ways to work with CFT

Cryo-Fluorescence Tomography Instrumentation & Services

Services

CFT fee-for-service research

EMIT Imaging offers CFT fee-for-service research performed in-house, at our state-of-the-art labs in Natick, MA and Owings Mills, MD. Simply freeze samples, ship them directly to us, and let EMIT handle the rest.

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Instrumentation

Xerra™ imaging platform

Xerra™ is EMIT Imaging's flagship imaging platform. Xerra fully automates the CFT workflow, enabling you to run samples in-house with ease, and provides unmatched visualization of on and off target drug distribution and protein expression in whole-animals.

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The technology

What is Cryo-Fluorescence Tomography?

Cryo-Fluorescence Tomography (CFT) is a transformative 3D approach to imaging drug distribution and protein expression in whole animals. EMIT Imaging offers both instrumentation and services via our imaging platform, Xerra™, a high resolution and high sensitivity preclinical imaging tool designed to advance discoveries in biological and drug research.

CFT can be easily integrated into your existing in vivo imaging workflows, providing definitive anatomical and molecular insights into:

Whole-body drug distribution and delivery
Screening candidate drugs and delivery systems
Whole-body therapeutic protein expression
Multiplexed co-localization of drug with targets
On and off target tissue identification
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What is Cryo-Fluorescence Tomography video thumbnail

Watch: What is Cryo-Fluorescence Tomography?

Want to see how your own data could look? Explore the full range of CFT visualization solutions.

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Applications

Preclinical Research Application Areas

Drug Discovery & Delivery

CFT is an important tool to monitor drug distribution, pharmacokinetic, and pharmacodynamic studies.

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Oncology & Immunotherapy

CFT can play a key role for studying tumor models including microenvironments, tumor heterogeneity, metastatic spread, and expression of specific biomarkers.

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Gene & Cell Therapy

CFT and Xerra™, a 3D imaging technology, allows whole-body detection and visualization of vector biodistribution and vector-mediated gene expression, in high resolution and with high sensitivity.

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Neuroscience

The 3D CFT maps of fluorescence reporters can aid in visualization of cell tracking, drug delivery, and pharmacodynamics in the brain.

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Antibody-Drug Conjugates

CFT provides whole-body visualization of ADC biodistribution, helping characterize on and off target payload delivery and linker stability.

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Why CFT

Benefits of Cryo-Fluorescence Tomography

CFT expands preclinical imaging capabilities by bridging the gap between cellular microscopy (imaging small tissue volumes at high resolution) and current whole-body techniques such as MRI, PET, and SPECT (imaging larger tissue volumes with lower resolution and sensitivity).

Cryo-Fluorescence Tomography

CFT fluorescence 3D reconstruction
3D CFT
CFT anatomical white-light section
Anatomical 2D CFT
  • 3D anatomical + molecular
  • High resolution
  • High sensitivity

2D Fluorescence

2D fluorescence mouse image
  • 2D surface image
  • Low resolution
  • Limited anatomical info
  • Signal highly attenuated

SPECT/CT

SPECT/CT fusion mouse image
  • Low resolution
  • Limited anatomical info
  • Requires radioactivity
Image Gallery

See Cryo-Fluorescence Tomography in action

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JAX mouse brain panel, fluorescence imaging
JAX mouse brain panel, fluorescence imaging
NSCLC mCherry-labeled metastasis
NSCLC mCherry-labeled metastasis
AAV vector biodistribution, horizontal cross-section
AAV vector biodistribution, horizontal cross-section
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