Findings: Whole-body CFT imaging of marmosets dosed intravenously with AAV8 or AAV9 expressing tdTomato shows the full spatial pattern of expression, and CFT signal correlates with ddPCR measurements from the same animal.

Findings: Using 3D Cryo-Fluorescence Tomography, researchers visualized whole-body GFP expression across multiple neurotropic AAV serotypes, revealing remarkable expression throughout the central nervous system and peripheral tissues. By comparing spatial expression patterns across vector designs, researchers gain valuable insight into whole-body transgene expression at sub-organ level resolution, supporting the development of next-generation gene therapies.

Findings: Imaging of isolated tissue samples with CFT provides higher resolution than whole-body. In this example, a mouse head was intracerebrally injected with GFP-expressing cells and imaged at 20 µm.

Findings: GAD67-GFP (line G42) mice selectively express enhanced green fluorescent protein (EGFP) in the parvalbumin (Pv)-expressing subclass of basket interneurons (soma, dendrites, and axons) and also in putative presynaptic boutons.