32 publications featuring Cryo-Fluorescence Tomography
Fluorine-19 MRI signal phenotype in solid tumor following in situ perfluorocarbon cell labeling
Ahrens ET, Tang K, Mognol GP, Chen H, Leach BI, Davis A, Varner J. Scientific Reports (2026). doi:10.1038/s41598-026-54741-4
Role of Xerra
CFT was used to visualize the 3D intratumoral biodistribution of PFC-labeled phagocytes throughout entire excised tumor masses.
CFT Advantage
Enabled whole-tumor, volumetric mapping of labeled immune cell distribution.
Preclinical Applications
Tumor-associated macrophage (TAM) burden imaging and biodistribution mapping in a syngeneic breast cancer model.
Probes Used in Xerra™
Dual-mode perfluorocarbon (PFC) nanoemulsion labeled with DiI fluorescent dye (555 nm ex / 585 nm em).
Imaging Modalities
CFT; 19F/1H MRI; flow cytometry; 19F NMR spectroscopy.
Animal Model
Orthotopic syngeneic 4T1-Luc2 breast tumor mouse model (female BALB/c mice).
Zwitterionic Modification of PSMA Ligands Reduces Off-Target Binding and Tissue Retention
Spickschen LFV, Thünauer R, Swierzewski AJ, et al. Journal of Medicinal Chemistry. 2026. doi:10.1021/acs.jmedchem.5c03849
Role of Xerra
Enabled high-resolution 3D visualization of fluorescent PSMA-targeted ligand biodistribution across whole mice using CFT, allowing precise localization of drug uptake and off-target retention.
CFT Advantage
Whole-animal imaging of fluorescent PSMA ligand distribution, revealing differences in tissue retention and organ localization between conventional and zwitterionically modified probes.
Preclinical Applications
Evaluation and optimization of PSMA-targeted ligands for prostate cancer imaging; characterization of tumor targeting and reduction of off-target accumulation.
Probes Used in Xerra™
Fluorescent PSMA ligand conjugates incorporating Cy5 or ZW800-1 dyes with zwitterionic linker modifications.
Imaging Modalities
CFT, widefield fluorescence microscopy, confocal fluorescence microscopy.
Animal Model
Mouse xenografts bearing PSMA-positive PC3-PIP and PSMA-negative PC3-flu prostate tumors.
Divergent Role of CD8 T Cells with Distinct Metabolic Phenotypes During Curative Radio-Immunotherapy in Hot versus Cold Tumors
Heaton AR, Burkard NJ, Gao A, et al. bioRxiv. 2026. doi:10.1101/2025.10.24.684274
Role of Xerra
Enabled 3D whole-body visualization of fluorescent CD8 T cell biodistribution and immune infiltration patterns across tumors and lymphoid tissues using CFT.
CFT Advantage
Unbiased, whole-animal visualization of immune cell localization.
Preclinical Applications
Immuno-oncology therapy evaluation; immune cell infiltration and tumor microenvironment; radio-immunotherapy response in hot versus cold tumors.
Probes Used in Xerra™
CD8-mCherry reporter mice enabling fluorescent tracking of CD8 T cells.
Imaging Modalities
CFT, intravital multiphoton optical metabolic imaging (OMI), multiplex immunofluorescence (mIF), flow cytometry, scRNAseq.
Animal Model
CD8-mCherry reporter mice bearing intradermal MC38 colon carcinoma (hot) or B78 melanoma (cold) tumors.
A Modular Platform for Enhanced Drug Delivery to Glioblastoma Using Targeted Multidomain Protein Assemblies
Angew. Chem. Int. Ed. 2025, e202503085. doi:10.1002/anie.202503085
Role of Xerra
Enabled high-resolution 3D visualization of NIR-labeled TMPA biodistribution in whole mice using CFT, allowing precise localization of drug delivery within brain tumor regions.
CFT Advantage
Whole-animal imaging of protein-based nanocarrier distribution, revealing preferential tumor accumulation and spatial localization within the brain.
Preclinical Applications
Evaluation and optimization of protein-based drug delivery systems for glioblastoma.
Probes Used in Xerra™
Near-infrared (NIR) dye-labeled targeted multidomain protein assemblies (TMPA), including TMPA loaded with doxorubicin (TMPA•Dox).
Imaging Modalities
CFT, ex vivo fluorescence imaging, pharmacokinetic blood analysis.
Animal Model
Orthotopic U87 glioblastoma mouse model.
A Preliminary Study on the Accuracy of MRI-Guided Thalamic Infusion of AAV2-GFP and Biodistribution Analysis Using CFT in Nonhuman Primates
Salegio EA, Espinosa R, Smith GR, et al. Pharmaceutics 17, 1167 (2025). doi:10.3390/pharmaceutics17091167
Role of Xerra
Provided automated, high-resolution 3D imaging to map whole-brain AAV2-GFP biodistribution in primates.
CFT Advantage
Sub-organ resolution, unbiased visualization of gene expression beyond MRI or histology limits.
Preclinical Applications
Assessment of targeted gene delivery accuracy and distribution in translational CNS therapy models.
Probes Used in Xerra™
AAV2 vector encoding GFP under CMV promoter, co-infused with gadoteridol.
Imaging Modalities
MRI-guided infusion with CFT and confirmatory histological staining.
Animal Model
Two adult African green monkeys received bilateral intrathalamic AAV2-GFP infusions under MRI navigation.
Preclinical Fluorescence-Guided Imaging Leveraging Surrounding Sentinel Tumor Microenvironment Identifies High-Risk Premalignant Pancreatic Lesions
Sharma S, Wen X, Wang J, et al. Clin Cancer Res (2025). doi:10.1158/1078-0432.CCR-25-1092
Role of Xerra
Enabled high-resolution NIR fluorescence imaging of tumor-associated inflammation.
CFT Advantage
Clear demarcation of premalignant lesions from background pancreatitis; sensitive early detection.
Preclinical Applications
Detection and surgical guidance of high-risk pancreatic lesions; evaluation of TME inflammation.
Probes Used in Xerra™
V-1520 (TSPO-targeted near-infrared fluorescent probe).
Imaging Modalities
CFT, in vivo fluorescence-guided imaging, confocal microscopy.
Animal Model
Kras G12D/+; Smad4 flox/flox (KSC) genetically engineered mouse model of pancreatic neoplasia.
A multimodal approach to assess a liposomal carrier's biodistribution, stability, and clearance
Wood CA, Jones CE, Jeevarathinam AS, et al. Photoacoustics 46, 100763 (2025).
Role of Xerra
Endpoint whole-mouse CFT at various timepoints to localize and quantify ICG fluorescence as a surrogate of liposome degradation and payload release; volumetric intensity analysis of liver, spleen, gallbladder, fecal matter.
CFT Advantage
High-resolution, 3D whole-body biodistribution of ICG with anatomical context, complementing photoacoustic; visualized biliary clearance and late splenic accumulation.
Preclinical Applications
Localization, probe biodistribution, stability, and clearance.
Probes Used in Xerra™
EGFR-Lipo-JICG & ICG.
Imaging Modalities
CFT, photoacoustic.
Animal Model
Athymic NCr nude mice.
![PREPRINT: A translatable evaluation tool to study the biodistribution of clinically-available doxorubicin liposomes: PET imaging of [89Zr]Zr-Doxil and [89Zr]Zr-Talidox](https://emitimaging.com/wp-content/uploads/2025/09/Shadow_A-Translateable_resized_500x500_cropped400-234x300.webp)
PREPRINT: A translatable evaluation tool to study the biodistribution of clinically-available doxorubicin liposomes: PET imaging of [89Zr]Zr-Doxil and [89Zr]Zr-Talidox
Carrascal-Miniño A, Mishra A, Gawne P, et al. bioRxiv (2025). doi:10.1101/2025.06.17.660144
Role of Xerra
CFT provided high-resolution whole-body imaging to complement PET quantification, highlighting off-target uptake and tissue distribution.
CFT Advantage
High-resolution 3D drug localization; reveals uptake heterogeneity beyond PET alone.
Preclinical Applications
Tumor localization; liposome biodistribution.
Probes Used in Xerra™
[89Zr]Zr-Doxil; [89Zr]Zr-Talidox; intrinsic doxorubicin fluorescence.
Imaging Modalities
CFT, PET/CT, autoradiography.
Animal Model
Female BALB/c mice bearing 4T1 mammary-pad tumors.
Development of an H&E on-block staining technique for collagen detection in cryofluorescence tomography imaging of frozen breast tissue samples
Snoddy EP, Tang TT, Fowlkes N, et al. PLOS One. 2025. doi:10.1371/journal.pone.0324493
Role of Xerra
Visualized collagen-associated autofluorescence and stromal anatomy in frozen breast tissue.
CFT Advantage
Whole-sample anatomical and fluorescence imaging with co-registered white-light and GFP data for localization of collagen-rich structures at sub-organ resolution.
Preclinical Applications
Evaluation of breast stromal architecture and improved region-of-interest selection for imaging-to-histology correlation.
Probes Used in Xerra™
Endogenous collagen autofluorescence via GFP fluorescence imaging (ex 470 nm, em 500 nm).
Imaging Modalities
CFT, GFP fluorescence imaging, stereo microscopy, conventional H&E histology.
Animal Model
Frozen human breast tissue specimens from bilateral mastopexy procedures.
Targeted suppression of type 1 interferon signaling during RNA delivery enhances vaccine-elicited immunity
Kim BJ, Hosn RR, Remba TK, et al. bioRxiv (2025). doi:10.1101/2025.03.09.642244
Role of Xerra
CFT was used to study mCherry expression volumes at injection sites.
CFT Advantage
Whole-tissue volumetric readout; sensitively detects expression increases with siIFNAR1.
Preclinical Applications
Injection-site localization & transgene biodistribution.
Probes Used in Xerra™
RepRNA encoding mCherry; siRNA to Ifnar1; mCherry fluorescence/LNP signal.
Imaging Modalities
CFT & fluorescence microscopy.
Animal Model
Balb/c mice; intramuscular gastrocnemius immunization.
PET imaging for non-invasive monitoring of 89Zr-Talidox delivery to the brain following focused ultrasound-mediated blood-brain barrier opening
Mishra A, Payne C, Carrascal-Miniño A, et al.
Role of Xerra
CFT was used alongside PET to study brain delivery of liposomal drug carriers across the blood-brain barrier after focused ultrasound; confirmed nanoscale probe distribution beyond PET resolution.
CFT Advantage
Revealed sub-organ biodistribution patterns complementing PET quantification.
Preclinical Applications
Brain localization & liposome biodistribution.
Probes Used in Xerra™
89Zr-Talidox & intrinsic doxorubicin fluorescence.
Imaging Modalities
CFT, PET, FUS.
Animal Model
Female BALB/c mice.
Mouse Cardiovascular Imaging
Phoon CK, Aristizábal O, Farhoud M, Turnbull DH, Wadghiri YZ. Current Protocols, 4, e1116 (2024). doi:10.1002/cpz1.1116
Role of Xerra
Comprehensive review of imaging approaches for evaluating cardiovascular structure and function in mouse models, with emphasis on echocardiography and MRI alongside CFT.
CFT Advantage
Whole-body, sub-organ level (~20-55 µm) visualization of molecular and anatomical signals, overcoming field-of-view, depth, or anatomical-context limits.
Preclinical Applications
Cardiovascular disease modeling: myocardial infarction, hypertrophy, heart failure, and developmental biology.
Imaging Modalities
CFT, echocardiography (high-frequency ultrasound), MRI, micro-CT, PET.
Animal Model
Mouse models for cardiovascular research, including genetically modified and disease models.
Comparison of Two Chelator Scaffolds as Basis for Cholecystokinin-2 Receptor Targeting Bimodal Imaging Probes
Gariglio G, Bendova K, Hermann M, et al. Pharmaceuticals (2024). doi:10.3390/ph17121569
Role of Xerra
CFT was used at the 72-hour timepoint to validate tumor-specific accumulation of the imaging probes in A431-CCK2R xenografts and differentiate off-target renal retention between the two chelators.
CFT Advantage
High-resolution, 3D volumetric imaging at late timepoints, complementing PET/fluorescence and enabling cross-validation of receptor-specific tumor targeting and off-target organ retention.
Preclinical Applications
Tumor targeting, biodistribution, dual-modality imaging validation, receptor-specific uptake.
Probes Used in Xerra™
[68Ga]Ga-CyTMG, [68Ga]Ga-CyFMG (SulfoCy5.5-labeled CCK2R-targeting dimers).
Imaging Modalities
CFT, PET, fluorescence imaging.
Animal Model
BALB/c mice with A431-CCK2R and A431-mock xenografts.
Cryo-Fluorescence Tomography as a Tool for Visualizing Whole-Body Inflammation Using Perfluorocarbon Nanoemulsion Tracers
Leach BI, Lister D, Adams SR, et al. Mol Imaging Biol (2024). doi:10.1007/s11307-024-01926-w
Role of Xerra
PFC nanoemulsions were visualized using CFT within tissues of the reticuloendothelial system and inflammatory lesions, consistent with immune cell (macrophage) labeling.
CFT Advantage
High-resolution, 3D mapping of probe localization in intact animals and tissue samples, enabling unbiased validation of biodistribution and minimizing reliance on histology.
Preclinical Applications
Localization, probe biodistribution.
Probes Used in Xerra™
NIR, red, and green PFC nanoemulsions.
Imaging Modalities
CFT.
Animal Model
C57BL/6 mice bearing MC38 tumors; 4T1 in Balb/c; CD-1 mice; wild-type C57BL/6 mice.
MRI-Based Cell Tracking of OATP-Expressing Cell Transplants by Pre-Labeling with Gd-EOB-DTPA
Bhattacharyya T, Mallett CL, Shapiro EM. Mol Imaging Biol 26, 233-239 (2024). doi:10.1007/s11307-024-01904-2
Role of Xerra
CFT showed that the areas of signal enhancement in MRI overlapped with areas of indocyanine green signal, indicating MRI signal enhancement was due to the transplanted cells.
CFT Advantage
High-resolution, high-sensitivity imaging with white-light anatomical data to help with sub-organ localization.
Preclinical Applications
Localization.
Probes Used in Xerra™
Indocyanine green.
Imaging Modalities
CFT, T1-weighted MRI.
Animal Model
HEK293 cells stably transduced to express human OATP1B3, injected into chicken hearts.
Intratumoral Biosynthesis of Gold Nanoclusters by Pancreatic Cancer to Overcome Delivery Barriers to Radiosensitization
Schwartz-Duval AS, Mackeyev Y, Mahmud I, et al. ACS Nano 2024, 18, 3, 1865-1881. doi:10.1021/acsnano.3c04260
Role of Xerra
CFT showed fluorescence from gold nanoclusters (GNCs) in the Au3+-treated group absent in the untreated control; GNC fluorescence across tumor cross-sections indicated uniform distribution.
CFT Advantage
Ability to track fluorescently labeled gold nanoclusters with high resolution and sensitivity.
Preclinical Applications
Monitor and track uptake of GNCs into tumors.
Imaging Modalities
CFT, confocal fluorescence.
Animal Model
PANC1 tumor xenografts 48 h after intratumoral injection of 0.00 or 1.00 mM Au3+.
Characterization and biodistribution of under-employed gene therapy vector AAV7
Yost SA, Firlar E, Glenn JD, et al. Journal of Virology. 2023 Oct 16; doi:10.1128/jvi.01163-23
Role of Xerra
CFT of whole mice was performed using an Xerra imager 3 weeks after IV administration of AAV7 or AAV9 carrying an EGFP transgene, or co-administration of AAV7.CAG.EGFP and AAV9.CAG.tdTomato (2x10^14 GC/kg).
CFT Advantage
Supported biodistribution findings in the brain, showing weak fluorescence for both AAV7 and AAV9; a surprising finding was highly concentrated fluorescence in facial structures.
Preclinical Applications
Protein expression, biodistribution, drug development.
Probes Used in Xerra™
EGFP, tdTomato.
Imaging Modalities
CFT, cryo-electron microscopy, immunofluorescence.
Animal Model
Female C57Bl/6 mice aged 6-8 weeks, divided into five groups (n=3).
Whole CNS 3D Cryo-Fluorescence Tomography Shows CSF Clearance along Nasal Lymphatics, Spinal Nerves, and Lumbar/Sacral Lymph Nodes
Stokes C, White EF, Toddes S, Bens N, Kulkarni P, Ferris CF. J. Imaging 2023, 9, 45. doi:10.3390/jimaging9020045
Role of Xerra
CFT was used to follow the movement of tracers from the ventricular system of the brain down through the meningeal lining of the spinal cord and out to the spinal lymphatic nodes.
CFT Advantage
Enabled following the distribution, localization, and clearance of Qdots over the entire CNS.
Preclinical Applications
Biodistribution, localization, and clearance.
Probes Used in Xerra™
Qdot 605 ITK amino (PEG).
Imaging Modalities
CFT.
Animal Model
Male C57BL/J6 mice.
Volumetric imaging of optically cleared and fluorescently labeled animal tissue (VIOLA) for quantifying the 3D biodistribution of nanoparticles at cellular resolution in tumor tissue
Rakhilin N, Yang B, Spilker ME, et al. Journal of Controlled Release, Volume 354, 2023, 244-259. doi:10.1016/j.jconrel.2022.12.060
Role of Xerra
Imaged tumor specimens using CFT, which measured nanoparticle biodistribution across the entire thickness of the tumor specimen.
CFT Advantage
Not limited by the depth limitations experienced with VIOLA at 1 mm.
Preclinical Applications
Biodistribution.
Imaging Modalities
CFT, VIOLA.
Animal Model
KPC-GEM mouse alongside BALB/c mice for CT26 xenograft and EMT6 mammary gland models.
A Genetically Encoded Magnetic Resonance Imaging Reporter Enables Sensitive Detection and Tracking of Spontaneous Metastases in Deep Tissues
Nyström NN, McRae SW, Martinez FM, Kelly JJ, Scholl TJ, Ronald JA. Cancer Res 2023; 83(5): 673-685. doi:10.1158/0008-5472.CAN-22-2770
Role of Xerra
CFT was utilized to identify 3D detection of micrometastases of a Luc-1B3 mouse.
CFT Advantage
Ability to identify on- and off-target metastases in 3D with high resolution and sensitivity.
Preclinical Applications
Identify and confirm location of metastases within mouse models.
Imaging Modalities
CFT, BLI, T1-weighted MRI, histology.
Animal Model
Luc-1B3 mouse burdened with lung micrometastases.
Biodistribution and delivery of oligonucleotide therapeutics to the central nervous system: Advances, challenges, and future perspectives
Goto A, Yamamoto S, Iwasaki S. Biopharmaceutics and Drug Disposition, 44(1), 26-47 (2023). doi:10.1002/bdd.2338
Role of Xerra
Review article comparing imaging modalities for analyzing biodistribution.
CFT Advantage
Whole-animal volumetric tissue imaging technique to monitor on- and off-target biodistribution.
Imaging Modalities
CFT, PET/SPECT, in vivo confocal microscopy, LSFM.
Preclinical characterization of novel radiolabeled and fluorescent-labeled Fibroblast Activation Protein (FAP)-targeting ligands using gamma counting, SPECT imaging and CFT
Hesterman, Novicki, White, Stokes, Cordova, Toddes, et al. EANM 2023
Role of Xerra
Visualize the biodistribution of the FAP imaging candidate compounds.
CFT Advantage
Higher-resolution whole-body information, yielding insights such as local distribution of the ligand on the surface of the bone and heterogeneity of distribution within the tumor.
Preclinical Applications
Tumor biology, biodistribution, drug development.
Probes Used in Xerra™
ZW800-1.
Imaging Modalities
SPECT/CT, CFT.
Animal Model
1 µg mass dose of [67Cu]RTX-1363 or [ZW800-1]RTX-1341 in U-87 MG tumor-bearing mice.
STING Agonist Delivery by Tumour-Penetrating PEG-Lipid Nanodiscs Primes Robust Anticancer Immunity
Dane EL, Belessiotis-Richards A, Backlund C, et al. Nature Materials. 21, 710-720 (2022)
Role of Xerra
High-resolution, high-sensitivity imaging of new LND-CDN carriers that are clinically viable immunostimulatory drugs to increase the response rate of immunotherapy.
CFT Advantage
3D validation of histology results; full-body biodistribution of agents.
Preclinical Applications
Tumor biology, biodistribution, drug development.
Probes Used in Xerra™
cGAMP-sulfo-Cy5.
Animal Model
B6 mice bearing MC38 flank tumours, administered LND or liposome labelled with 2.0 nmol sulfo-Cy5 via retro-orbital injection.
High-Contrast Detection of Somatostatin Receptor Subtype 2 for Fluorescence-Guided Surgery
Hernandez Vargas S, AghaAmiri S, Ghosh SC, et al. Mol. Pharmaceutics 2022, 19, 4241-4253
Role of Xerra
Map out the distribution and signal intensity of conjugates while maintaining anatomical context in a mouse tumor model.
CFT Advantage
High-resolution, high-sensitivity imaging of MMC(FNIRTag)-TOC and its IR800 counterpart in SSTR2+ and SSTR2- tumors.
Preclinical Applications
Imaging agent development, tumor biology.
Imaging Modalities
Bioluminescence, confocal microscopy, NIRF imaging, PET/CT.
Animal Model
Athymic female J:Nu nude mice with NEC913/NEC1452 xenografts.
Microenvironment-triggered multimodal precision diagnostics
Hao L, Rohani N, Zhao RT, et al. Nature Materials. 20, 1440-1448 (2021)
Role of Xerra
Detect the localization of fluorescent signal in a pattern aligned with the corresponding anatomical tumors throughout the lung after systemic administration of FAM-labelled PRISM.
CFT Advantage
Detected anatomical tumor location and a protease-responsive imaging sensor for malignancy (PRISM); analysis software created fly-through animations and 3D models.
Preclinical Applications
Oncology, drug discovery.
Probes Used in Xerra™
FAM fluorophore in PRISM (ex/em 470 nm / 511/20 nm).
Imaging Modalities
CFT, PET/CT, confocal microscopy, immunofluorescence.
Animal Model
Female BALB/c mice.
Emergent Fluorous Molecules and Their Uses in Molecular Imaging
Wang C, Adams SR, Ahrens ET. Accounts of Chemical Research. 2021 Aug 3;54(15):3060-3070
Role of Xerra
Visualize nanoemulsion (NE) biodistribution.
CFT Advantage
3D imaging of NE, registration of white-light and fluorescence signal, high sensitivity compared to other fluorescence imaging modalities.
Preclinical Applications
Gene therapy, oncology, neurology, immunology, drug discovery.
Probes Used in Xerra™
Cy5 (ex/em 673/707).
Imaging Modalities
CFT, 19F and 1H MRI, bimodal PET/MRI, PET, CT, IVIS.
Animal Model
Acute inflammation murine model.
Immunotherapy-induced antibodies to endogenous retroviral envelope glycoprotein confer tumor protection in mice
Kang BH, Momin N, Moynihan KD, Silva M, Li Y, Irvine DJ, et al. PLoS ONE 16(4): e0248903 (2021). doi:10.1371/journal.pone.0248903
Role of Xerra
Visualize 1E4 effectively targeting tumor cells.
CFT Advantage
Ability to detect a strong signal in the tumor, indicating 1E4 effectively targeted tumor cells.
Preclinical Applications
Biodistribution of 1E4 in a mouse bearing a B16F10 tumor.
Probes Used in Xerra™
Alexa Fluor 750 NHS Ester.
Imaging Modalities
CFT, 2D immunoblot.
Animal Model
Naive mouse bearing a B16F10 tumor, injected with 500 µg 1E4-AF750.
Transcriptomic Analysis of Diffuse Intrinsic Pontine Glioma (DIPG) Identifies a Targetable ALDH-Positive Subset of Highly Tumorigenic Cancer Stem-like Cells
Surowiec RK, Ferris SF, Apfelbaum A, et al. Mol Cancer Res 2021; 19(2): 223-239. doi:10.1158/1541-7786.MCR-20-0464
Role of Xerra
3D reconstructions of CFT imaging demonstrated diffuse tumor cell infiltration in implanted brains, most densely populated near the implantation site (pons) and becoming more diffuse through the midline and frontal cortex.
CFT Advantage
Cell localization of tumor cell infiltration in the implanted brains.
Preclinical Applications
Neurology.
Probes Used in Xerra™
mCherry.
Imaging Modalities
CFT, histology, BLI.
Animal Model
Murine brain from mCherry-labeled DIPG 007 ALDH+ implanted cells, removed on day 91.
Fluorescence-guided resection of tumors in mouse models of oral cancer
Dem-trio de Souza França P, Guru N, Roberts S, et al. Sci Rep 10, 11175 (2020). doi:10.1038/s41598-020-67958-8
Role of Xerra
A mouse with oral cancer and palpable bilateral neck metastases, along with a control mouse, were imaged to evaluate PARPi-FL uptake.
CFT Advantage
Tumor localization.
Preclinical Applications
Oncology.
Probes Used in Xerra™
PARPi-FL.
Imaging Modalities
CFT, epifluorescence imaging, stereoscope imaging, confocal microscopy.
Animal Model
C57BL/6J black mice, athymic nude mice.
Fluorescence labeling of a NaV1.7-targeted peptide for near-infrared nerve visualization
Gonzales J, Pirovano G, Chow CY, et al. EJNMMI Res 10, 49 (2020). doi:10.1186/s13550-020-00630-4
CFT Advantage
High-resolution biodistribution.
Preclinical Applications
CNS.
Probes Used in Xerra™
Hs1a-FL.
Imaging Modalities
CFT, confocal microscopy, epifluorescence imaging.
Animal Model
Female athymic nude mice.
Brain Pharmacology of Intrathecal Antisense Oligonucleotides Revealed Through Multimodal Imaging
Mazur C, Powers B, Zasadny K, et al. JCI Insight. 2019 Oct 17;4(20)
Role of Xerra
To define 2D and 3D spatiotemporal pharmacokinetics of ASO penetration in CNS structures and demonstrate their interaction with multiple molecular movement paths.
CFT Advantage
2D/3D imaging of ASO brain penetration, registration of white-light and fluorescence signal, and collection of brain sections on glass slides for fluorescence microscopy and IHC.
Preclinical Applications
Neurology, drug discovery.
Probes Used in Xerra™
Cy7 (ex/em 756/779).
Imaging Modalities
CFT, SPECT/CT, 18F PET, microscopy.
Animal Model
Sprague Dawley rats.
Dynamic Dual-Isotope Molecular Imaging Elucidates Principles for Optimizing Intrathecal Drug Delivery
Wolf DA, Hesterman JY, Sullivan JM, et al. JCI Insight. 2016 Feb 25;1(2)
Role of Xerra
To determine the effects of neuraxial exposure of drug surrogate molecules following an IT lumbar bolus in the rat.
CFT Advantage
CFT was used to follow the IT distribution of EBD, a small molecule (MW 961 Da) that binds tightly to albumin; analysis software created fly-through animations and 3D models and estimated CSF volume.
Preclinical Applications
Neurology, drug discovery.
Probes Used in Xerra™
Cy5 (ex/em 673/707).
Imaging Modalities
MRI, SPECT/CT, autoradiography, microscopy.
Animal Model
Sprague Dawley rats.