Drug discovery & delivery
Monitor on- and off-target expression with resolution and sensitivity.
CFT for drug discovery and delivery
Cryo-Fluorescence Tomography (CFT) is an ex vivo, volumetric tissue imaging technique used to monitor the pharmacokinetics and pharmacodynamics (PK/PD) of drugs and delivery vehicles with high resolution and sensitivity. Images of the anatomy (white light) are acquired alongside fluorescence images that are displayed in a 3D visualization of the drug and/or delivery vehicle biodistribution.
EMIT Imaging's visualization tools make CFT data easier to explore and collaborate on, with a full range of options to fit different user types and use cases.
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AAV-mediated protein expression
Findings: CFT uniquely achieves the required combination of resolution and sensitivity in a whole animal.
- GFP expression at day 15 following IV injection
- No expression in the control
- Liver is the major organ where GFP expression occurs, but protein expression is observed throughout the body
- Note signal in dorsal root ganglion (DRG) and heart, consistent with AAV9 tropism
Visualization of cell intake
Findings: CFT was used to visualize labeled cell intake into tissue.
- Cell tracking by fluorescence labeling is a critical aspect of cell-based therapies, such as CAR-T and regenerative medicine
- In preclinical research, cells are administered systemically or directly in target tissues, and localization is evaluated using in vivo or tissue imaging
- In this example, proprietary cells were labeled with commercial QTracker 800 (ThermoFisher) and injected into mouse spleen
- Primary pellet observed in the spleen and a secondary pellet in the liver
CFT application in large animal tissue
Findings: Because Xerra works in large formats, CFT has strong applications for anatomical RGB white-light and fluorescence imaging in large animal tissue, including NHP, bovine, porcine, and more.
White-lightFluorescence
White-lightFluorescence