Oncology and Immunotherapy
Monitor Whole-Body Drug Biodistribution in High Resolution and High Sensitivity
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CFT in Oncology and Immunotherapy Research

Cryo-Fluorescence Tomography (CFT) and EMIT Imaging’s flagship platform Xerra™ are powerful techniques for oncology and immunotherapy applications. CFT provides anatomical, RGB white-light images & 3D fluorescence images to helps researchers to discover more when studying tumor models including microenvironments, tumor heterogeneity, metastatic spread, and expression of specific biomarkers.


For metastatic tumor progression, CFT detects metastatic disease and provides high resolution 3D molecular data of tumor burden and spread, not typically visualized in other image modalities. In addition CFT is used to evaluate tumor metabolism and cell biology in response to genetic manipulations, pharmacologic agents, and cancer chemotherapy drugs.

Example: Whole Body Drug Biodistribution

Fibroblast-Activation Protein

Findings: Data confirms drug preferential uptake in the tumor with minimal uptake in other organs/tissues

 

Details: 

  • ZW800-1-labeled FAP-targeting small molecule
  • U87 xenograft
  • 4hrs post iv administration
  • Imaged at 35 µm

Copyright 2023 Ratio Therapeutics Inc., used with permission

3D Fluorescence
Anatomical White-light
Fluorescence
Anatomical + Fluorescence
1. Tumor heterogeneity; 2. Signal in humerus: On-target signal in joints due to FAP-expression in juvenile mice
3. Signal in femoral head: On-target signal in joints due to FAP-expression in juvenile mice

Example: Co-localization of Tumor and Drug

Comparison of FLI and CFT

Findings: CFT shows high-resolution, 3D co-localization of tumor cells and drugs, complementing and expanding 2D in vivo imaging

 

Details

  • Orthotopic ovarian cancer model with tdTomato-expressing cells
  • Injected HER2-targeted antibody labeled with AF647

Ovarian Cancer cells (tdTomato)

FLI
3D CFT

Her2-Targeting Protein (AF647)

FLI
3D CFT

Example: Understanding of Disease Models

Comparison of BLI and CFT

Findings: CFT image shows the primary tumor extent and heterogeneity as well as the metastatic lesions in the lungs

 

Details:

  • MDA-MB-231 human breast adenocarcinoma cells were engineered to express both firefly luciferase and DsRed
  • The in vivo bioluminescence image shows the flank tumor and can be used to track progression

DsRed mice provided by John Ronald, Robarts Research Institute

BLI (ffluc)

CFT (DsRed)

Discover More with Cryo-Fluorescence Tomography