Gene & Cell Therapy
Visualization of vector biodistribution and vector-mediated gene expression
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CFT in Gene and Cell Therapy

New vectors are in development to improve their efficacy, including biodistribution and transduction, while minimizing off-target or safety liabilities. Visualization of vector biodistribution and subsequent gene transduction in preclinical models are crucial in early-stage research; however, studies are challenging to do using existing imaging modalities.


See what you’re missing with Cryo-Fluorescence Tomography (CFT) and Xerra™, a 3D imaging technology that allows whole-body detection and visualization of vector biodistribution and vector-mediated gene expression, in high resolution and with high sensitivity.

AAV-mediated Protein Expression Following IV Administration

Example: Whole Body Distribution of Labeled ASO

Intrathecal Injection

Findings: CFT is superior to surface fluorescence due to its depth limitations (signal loss) and poor resolution


  • Cy5-labeled ASO at day 15 following IT injection
  • Full visualization of spinal column into the cisterna magna
  • CFT detects bioD and clearance: GI, lympathic, nasal turbinate, etc.
  • CFT is 3D and high resolution: <50mm versus >1mm in optical and nuclear medicine imaging

–> Signal loss due to depth, note the spine in CFT

-> Site of Injection

2D In Vivo Fluorescence (Prone)
2D In Vivo Fluorescence (Supine)

Example: Whole-Body Transduction

AAV7-GFP + AAV9-tdTomato

Findings: CFT visualization of whole-body transduction and expression by AAV9 and AAV7



  • Representative images of animals administered single vectors or both AAV7.CAG.EGFP and AAV9.CAG.tdTomato. (A,B)
  • Representative CFT images of EGFP fluorescence in hind limb muscle (C) and protein copy number by qPCR (D)

Yost et. al, ASGCT 2023

Example: Regional Transduction

AAV7-GFP + AAV9-tdTomato

Findings: CFT identified several tissues with AAV transduction and protein expression




Widespread transduction by both AAV7 (A) and AAV9 (B) in the muscle cells of the tongue, tissues in the sinus cavity, lacrimal and Harderian glands beneath the eye, and inside the marrow of the jaw and outside the roots of the incisors.

Yost et. al, ASGCT 2023

Discover More with Cryo-Fluorescence Tomography