Gene & Cell Therapy
Visualization of vector biodistribution and vector-mediated gene expression
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CFT in Gene and Cell Therapy

New vectors are in development to improve their efficacy, including biodistribution and transduction, while minimizing off-target or safety liabilities. Visualization of vector biodistribution and subsequent gene transduction in preclinical models are crucial in early-stage research; however, studies are challenging to do using existing imaging modalities.

See what you’re missing with Cryo-Fluorescence Tomography (CFT) and Xerra™, a 3D imaging technology that allows whole-body detection and visualization of vector biodistribution and vector-mediated gene expression, in high resolution and with high sensitivity.

    Example: Whole Body Distribution of Labeled ASO

    Intrathecal Injection

    Findings: CFT is superior to surface fluorescence due to its depth limitations (signal loss) and poor resolution


    • Cy5-labeled ASO at day 15 following IT injection
    • Full visualization of spinal column into the cisterna magna
    • CFT detects bioD and clearance: GI, lympathic, nasal turbinate, etc.
    • CFT is 3D and high resolution: <50mm versus >1mm in optical and nuclear medicine imaging

    –> Signal loss due to depth, note the spine in CFT

    -> Site of Injection

    2D In Vivo Fluorescence (Prone)
    2D In Vivo Fluorescence (Supine)
    3D CFT

    Example: Whole-Body Transduction

    AAV7-GFP + AAV9-tdTomato

    Findings: CFT visualization of whole-body transduction and expression by AAV9 and AAV7



    • Representative images of animals administered single vectors or both AAV7.CAG.EGFP and AAV9.CAG.tdTomato. (A,B)
    • Representative CFT images of EGFP fluorescence in hind limb muscle (C) and protein copy number by qPCR (D)

    Yost et. al, ASGCT 2023

    Example: Regional Transduction

    AAV7-GFP + AAV9-tdTomato

    Findings: CFT identified several tissues with AAV transduction and protein expression




    Widespread transduction by both AAV7 (A) and AAV9 (B) in the muscle cells of the tongue, tissues in the sinus cavity, lacrimal and Harderian glands beneath the eye, and inside the marrow of the jaw and outside the roots of the incisors.

    Yost et. al, ASGCT 2023

    Discover More with Cryo-Fluorescence Tomography