Gene & cell therapy
Visualization of vector biodistribution and vector-mediated gene expression.
CFT in gene and cell therapy
New vectors are in development to improve their efficacy, including biodistribution and transduction, while minimizing off-target or safety liabilities. Unbiased visualization of vector biodistribution and subsequent gene transduction in preclinical models is crucial in early-stage research; however, these critical studies are challenging using standard imaging modalities.
See what you're missing with Cryo-Fluorescence Tomography (CFT) and Xerra™, a 3D imaging technology that allows whole-body detection and visualization of vector biodistribution and vector-mediated gene expression, in high resolution and with high sensitivity.
EMIT Imaging's visualization tools make CFT data easier to explore and collaborate on, with a full range of options to fit different user types and use cases.
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Whole-body AAV biodistribution in non-human primates
Findings: Whole-body CFT imaging of marmosets dosed intravenously with AAV8 or AAV9 expressing tdTomato shows the full spatial pattern of expression, and CFT signal correlates with ddPCR measurements from the same animal.
Neurotropic AAV serotypes
Findings: Using 3D Cryo-Fluorescence Tomography, researchers visualized whole-body GFP expression across multiple neurotropic AAV serotypes, revealing remarkable expression throughout the central nervous system and peripheral tissues. By comparing spatial expression patterns across vector designs, researchers gain valuable insight into whole-body transgene expression at sub-organ level resolution, supporting the development of next-generation gene therapies.
Cardiac specificity of AAV capsid and promoter pairs
Findings: Using 3D Cryo-Fluorescence Tomography, whole-body mCherry expression was visualized in mice dosed with AAVs to confirm cardiac specificity and to assess potential toxic distribution to the central nervous system for cardiac-specific capsid and promoter pairs. The resulting 3D datasets show expression in the heart alongside transgene expression throughout the rest of the body, including the central nervous system, at sub-organ level resolution.
AAV-mediated protein expression
Findings: 3D CFT visualization of AAV vector biodistribution and expression following IV administration.
Whole-body distribution of labeled ASO
Findings: CFT enhances data from 2D in vivo FLI by addressing its depth and resolution limitations.
Whole-body transduction
Findings: CFT visualization of whole-body transduction and expression by AAV9 and AAV7.
Regional transduction
Findings: CFT identified several tissues with AAV transduction and protein expression.
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Open the cardiac or neurotropic AAV dataset, or the multiplexed vehicle and cargo example, and page through slices, toggle fluorescence channels, and rotate the reconstructed volume. Nothing to download and nothing to install.