The Role of Cryo-Fluorescence Tomography in Analyzing AAV7 and AAV9 Whole-Body Vector Biodistribution
This case study highlights the role of EMIT Imaging and CFT in analyzing AAV7 and AAV9 whole animal drug biodistribution. The data, published by REGENXBIO in the Journal of Virology, explored the potential of AAV7 as a gene therapy vector and compared its biodistribution with AAV9 in mice following intravenous administration. CFT was used extensively to analyze whole-body vector biodistribution and to guide IHC and PCR methods.
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Analyzing the Biodistribution of a Fibroblast Activation Protein Imaging Compound with Cryo-Fluorescence Tomography
This case study highlights the role of EMIT Imaging and CFT in analyzing the biodistribution of a Fibroblast Activation Protein (FAP) imaging compound. Researchers compared biodistribution in U-87 MG tumor-bearing mice using a ZW800-1 fluorescent-labeled FAP compound and a 67Cu-labeled NOTA-conjugated FAP compound of analogous structure. Drug uptake was measured from regions of interest across tumor, knee joint, liver, and kidney, and CFT was benchmarked against SPECT and gamma counting.
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Cryo-Fluorescence Tomography Outperforms 2D In Vivo Fluorescence Imaging
This case study shows how 2D in vivo fluorescence imaging (FLI) failed to accurately localize compounds in complex biological models, leading to misinterpretations. By integrating high resolution, 3D imaging through CFT, the study revealed critical anatomical and molecular insights missed by traditional optical methods, correcting initial misinterpretations in a fibrin-targeting drug study and demonstrating CFT's advantage in sensitivity, resolution, and anatomical context.
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