Cryo-Fluorescence Tomography Services

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Cryo-Fluorescence Tomography Services

For researchers looking to discover the benefits of CFT without purchasing a system, EMIT Imaging offers end-to-end preclinical services performed at our state-of-the-art facilities in Boston, MA and Baltimore, MD. The process is seamless and easy: freeze whole-animal/tissue samples, ship them to us, and let EMIT handle the rest. Our experienced imaging scientists work with you hand-in-hand to ensure studies are designed to provide unmatched visualization of drug PK/PD. See what you’re missing compared to existing fluorescence imaging techniques and utilize CFT to gain better insights in 3D and with high-resolution and high-sensitivity. Complete services include:

 

  • Scientific expertise to support study design and execution
  • Dedicated project manager from start to finish
  • Fast turnaround times: Initial data in as soon as 14 days
  • Advanced data analysis

How CFT Works

Step 1: Preparation

Cryo-preserve & embed the sample in OCT

Step 2: Image + Section

High resolution, high sensitivity white light and fluorescence images are acquired. The block is sectioned and 20-55 microns are removed from the block surface. Process is repeated until the whole sample is sectioned and imaged.

Step 3: Output

3D image stacks are generated. White light and fluorescence data can be overlaid.

CFT Research Advantages

High Resolution:

High, near-cellular resolution down to 20 µm

High Sensitivity:

Nanomolar sensitivity, comparable to nuclear medicine

High Throughput:

Several samples in the same block = large volume of tissue imaged

Complementary

Fits into any experiment, complementing tissue and radiology imaging

Simplified:

No fixation, perfusion, clearing or radiolabeling required

Multiplexing:

6 lasers and 7 filters for multiplexed applications

Discover More with Cryo-Fluorescence Tomography