The ability to engineer mouse models that represent human disease is a valuable tool used by research groups around the world to better understand the biology of disease and drug targeting characteristics. A gap in sensitivity and resolution between in vivo and ex vivo techniques may make it hard to characterize an animal model. Using Cryo-Fluorescence Tomography (CFT), researchers can now obtain a 3D rendering of their mouse model to precisely understand the anatomical location of their genes of interest.
CFT – as high-res as it gets
Fluoro-tagged adeno-associated viruses (AAVs) are used to deliver genes to mammalian cells and express fluorescent marker proteins in animals. Cryo-fluorescence tomography (CFT) quantifies this fluorescence, and differentiates different AAV strains, across the whole animal.
High-resolution localization of liver markers was achieved with CFT.
AAV liver markers
BLI and CFT image of same tumor mouse model.
CFT image showing metastasis to the abdomen, lung, and bone not previously detected by bioluminescence
4T1 mouse mammary tumor cell line in immunodeficient mice.